Construction of an Eukaryotic Expression Vector Encoding Herpes Simplex Virus Type 2 Glycoprotein D and In Vitro Expression of the Desired Protein

construction of an eukaryotic expression vector encoding herpes simplex virus type 2 glycoprotein d and in vitro expression of the desired protein

to construct of an eukaryotic expression vector encoding herpes simplex virus type 2 (hsv-2) glycoprotein d (gd2), an iranian isolate of hsv-2 was propagated in hela cell line and its dna was extracted and used as template in polymerase chain reactions (pcr), to amplify gd2 gene. primers were designed and the restriction enzyme sites for ecori and xhoi were considered at their 5′ ends respectiv...

Expression of the Herpes Simplex Virus Type 2 Glycoprotein D in Baculovirus Expression System and Evaluation of Its Immunogenicity in Guinea Pigs

Background: Herpes simplex virus type 2 (HSV-2) is highly prevalent and major cause of genital herpes in humans. The life-long nature of infection and the increasing prevalence of genital herpes imply that vaccination is the best strategy for controlling the spread of infection and limiting HSV disease. HSV glycoprotein D (gD) is one of the most important viral immunogen which has an essential ...

Expression and in vitro Characterization of Herpes Simplex Virus 1 (HSV-1) ORF P Protein

Herpes simplex virus 1 (HSV-1) unspliced 8.3 latency associated transcript (LAT), which located in the long repeat sequences, has been shown to contain at least 16 open reading frames (ORF: A-P). One of these ORF, ORF P, maps almost entirely antisense to HSV-1 neurovirulence gene, ICP34.5. Both ORF P and ICP34.5 are located in the long repeat and are antisense overlapping genes. Therefore, in O...

Immunogenicity and Efficacy of Baculovirus Derived Glycoprotein D of Herpes Simplex Virus Type-I in Mice

Expression, Purification, and Antiserum Production of the Truncated UL31 Protein of Herpes Simplex Virus 1

Background: The UL31 protein of herpes simplex virus 1 (HSV-1) plays an important role in the HSV-1 replication, however, its pinpoint functions in the life cycle of the virus have yet to be adequately elucidated. Objectives: An antiserum specific for detecting HSV-1 UL31 was prepared as the foundation for future research on the role of UL31 in the course...

expression of the herpes simplex virus type 2 glycoprotein d in baculovirus expression system and evaluation of its immunogenicity in guinea pigs

background: herpes simplex virus type 2 (hsv-2) is highly prevalent and major cause of genital herpes in humans. the life-long nature of infection and the increasing prevalence of genital herpes imply that vaccination is the best strategy for controlling the spread of infection and limiting hsv disease. hsv glycoprotein d (gd) is one of the most important viral immunogen which has an essential ...